Waller M, Normansell D E
Am J Clin Pathol. 1975 Sep;64(3):358-64. doi: 10.1093/ajcp/64.3.358.
Small amounts of subtilisin (0.1 mg. per ml.) in the absence of cysteine will instantaneously hydrolyze "enzyme-sensitive" IgG globulins. This procedure permits the identification of antibodies in whole serum and myeloma globulins in the ultracentrifuge with simplicity and speed unmatched by other technics.
