Wolf H, Lang D M
Fakultät für Biologie, Universität Konstanz, Germany.
J Neurobiol. 1994 Jul;25(7):846-64. doi: 10.1002/neu.480250709.
In the primordial thoracic ganglia of locust embryos, the bromodeoxyuridine (BrdU) technique for labelling proliferating cells and their progeny was combined with intracellular dye injection to investigate the origin and the clonal relationship of common inhibitory motoneurons. Common inhibitors 1 (CI1) and 3 (CI3) were found to be siblings, that is, they are produced by the division of one ganglion mother cell. This ganglion mother cell results from the first division of neuroblast 5-5, at about 30% of embryonic development. A large portion, at least, of the ganglion mother cells produced by subsequent divisions of neuroblast 5-5 give rise to interneurons with contralaterally ascending or descending axons and GABA-like immunoreactivity. Thus, CI1 and CI3 are more closely related to putative inhibitory interneurons than they are to other, that is, excitatory, motoneurons. Consistent with this, the CI somata are associated with cell bodies of putative inhibitory interneurons rather than with clusters of excitatory motoneuron somata. These results elicit speculations regarding the evolutionary origin of inhibitory motoneurons.
在蝗虫胚胎的原始胸神经节中，将用于标记增殖细胞及其后代的溴脱氧尿苷（BrdU）技术与细胞内染料注射相结合，以研究共同抑制性运动神经元的起源和克隆关系。发现共同抑制神经元1（CI1）和3（CI3）是同胞，也就是说，它们由一个神经节母细胞分裂产生。这个神经节母细胞源于神经母细胞5-5在胚胎发育约30%时的第一次分裂。神经母细胞5-5随后的分裂产生的大部分神经节母细胞产生具有对侧上行或下行轴突以及GABA样免疫反应性的中间神经元。因此，CI1和CI3与假定的抑制性中间神经元的关系比它们与其他（即兴奋性）运动神经元的关系更为密切。与此一致的是，CI的胞体与假定的抑制性中间神经元的细胞体相关联，而不是与兴奋性运动神经元胞体的簇相关联。这些结果引发了关于抑制性运动神经元进化起源的推测。
