嗜热古菌延伸因子1α的结晶
Crystallization of a hyperthermophilic archaeal elongation factor 1 alpha.
作者信息
Zagari A, Sica F, Scarano G, Vitagliano L, Bocchini V
机构信息
Università di Napoli Federico II, Centro di Studio di Biocristallografia, CNR, Italy.
出版信息
J Mol Biol. 1994 Sep 16;242(2):175-7. doi: 10.1006/jmbi.1994.1568.
PMID:8089838
Abstract
Intact and fully active elongation factor aEF-1 alpha from the hyperthermophilic archaeon Sulfolobus solfataricus has been crystallized as a complex with GDP. Crystals were stable at temperatures below 8 degrees C and showed significant diffraction beyond 3.0 A. The orthorhombic lattice parameters were a = 62.9 A, b = 81.3 A, c = 115.6 A with one molecule per asymmetric unit.
摘要
来自嗜热古菌嗜热栖热菌的完整且具有完全活性的延伸因子aEF-1α已与GDP形成复合物结晶。晶体在8摄氏度以下的温度下稳定，并在3.0埃以上显示出明显的衍射。正交晶格参数为a = 62.9埃，b = 81.3埃，c = 115.6埃，每个不对称单元有一个分子。
Zotero 插件