Hamilton T E, Lawrence P J
Antimicrob Agents Chemother. 1975 Jul;8(1):38-44. doi: 10.1128/AAC.8.1.38.
Triton X-100 treatment or freeze-thawing damages the membranes of sporulating or vegetative cells as seen by protein leakage from cells. A 40% increase in the specific [(14)C]benzylpenicillin-binding capacity of detergent-treated or frozen sporulating cells was observed. Neither freezing nor Triton X-100 treatment of vegetative cells produced a detectable effect on their [(14)C]benzylpenicillin-binding capacity. These data indicate the presence of penicillin-binding sites in intact sporulating bacilli not accessible to penicillin in routine binding assays. The chemical specificity of [(14)C]benzylpenicillin binding to detergent-treated sporulating cells is similar to that observed with untreated vegetative or sporulating cells.
如细胞蛋白质渗漏所示，Triton X-100处理或冻融破坏了芽孢形成细胞或营养细胞的膜。观察到经去污剂处理或冷冻的芽孢形成细胞的比[(14)C]苄青霉素结合能力增加了40%。对营养细胞进行冷冻或Triton X-100处理均未对其[(14)C]苄青霉素结合能力产生可检测到的影响。这些数据表明，在完整的芽孢形成杆菌中存在常规结合试验中青霉素无法接近的青霉素结合位点。[(14)C]苄青霉素与经去污剂处理的芽孢形成细胞结合的化学特异性与未处理的营养细胞或芽孢形成细胞相似。
