Weber N, Vosmann K, Aitzetmüller K, Filipponi C, Taraschewski H
Institut für Biochemie und Technologie der Fette, BAGKF, Münster, Germany.
Lipids. 1994 Jun;29(6):421-7. doi: 10.1007/BF02537311.
The sterol composition of free sterol and steryl ester fractions of the fish parasite Paratenuisentis ambiguus was determined. In addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters, triacylglycerols and free fatty acids, as well as the composition of the 1-O-alkyl moieties of total ether glycerolipids of the parasite, were investigated. The results of these studies were compared with those obtained on the intestinal tract tissue of its host, the eel (Anguilla anguilla). Cholesterol is the major sterol in both P. ambiguus and A. anguilla. However, the sterols of P. ambiguus contain high proportions (> 20%) of other sterols, such as campesterol and various dehydrosterols. [e.g., 7-dehydrocholesterol and cholesta-5,22(E)-dienol]. The presence of these minor sterols agrees with the known biotransformations of exogenous sterols in various helminths. Considerable differences are found in the fatty acid composition of neutral lipid fractions, as well as the total lipid extract from the endoparasite as compared to the host tissue. In particular, eicosapentaenoic acid (20:5n-3), other polyunsaturated fatty acids, such as 20:4n-6, 22:5n-3 and 22:6n-3, as well as long-chain saturated fatty acids, such as 20:0, are generally enriched in the neutral lipid fractions of the parasite as compared to those of infected eel intestine.(ABSTRACT TRUNCATED AT 250 WORDS)
测定了鱼类寄生虫模糊副新棘虫游离甾醇和甾醇酯组分的甾醇组成。此外，还研究了各种中性脂质类别的脂肪酸组成，即蜡酯、甾醇酯、三酰甘油和游离脂肪酸，以及该寄生虫总醚甘油脂中1-O-烷基部分的组成。将这些研究结果与其宿主鳗鱼（欧洲鳗鲡）肠道组织的研究结果进行了比较。胆固醇是模糊副新棘虫和欧洲鳗鲡中的主要甾醇。然而，模糊副新棘虫的甾醇含有高比例（>20%）的其他甾醇，如菜油甾醇和各种脱氢甾醇[例如，7-脱氢胆固醇和胆甾-5,22（E）-二烯醇]。这些次要甾醇的存在与各种蠕虫中外源甾醇的已知生物转化一致。与宿主组织相比，在内寄生虫的中性脂质组分以及总脂质提取物的脂肪酸组成中发现了相当大的差异。特别是，与感染鳗鱼肠道相比，二十碳五烯酸（20:5n-3）、其他多不饱和脂肪酸，如20:4n-6、22:5n-3和22:6n-3，以及长链饱和脂肪酸，如20:0，通常在寄生虫的中性脂质组分中富集。（摘要截断于250字）
