Sul D, Kaneshiro E S, Jayasimhulu K, Erwin J A
Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, Chicago 60616, USA.
J Eukaryot Microbiol. 2000 Jul-Aug;47(4):373-8. doi: 10.1111/j.1550-7408.2000.tb00063.x.
The neutral lipids and their fatty acids and the sterol fractions of the marine ciliated protozoon, Parauronema acutum, were characterized. The neutral lipids consisted of triglycerides (30%), sterols (29%), free fatty acids (24%), steryl esters (9%), and diglycerides (8%) and small amounts of fatty alcohols. The fatty acid profiles of these lipids were very similar although quantitative differences were detected. Saturated fatty acids, primarily 14:0, 16:0, and 18:0 constituted 20-30% of the total. Unsaturated fatty acids containing one to three double bonds, primarily 18:1(9), 18:2 (9,12), 18:3 (9, 12, 15) and 20:3 (11, 14, 17), constituted 35-50% of the total. Highly unsaturated fatty acids, 18:4 (6, 9, 12, 15), 20:5 (5, 8, 11, 14, 17) and 22:6 (4, 7, 10, 16, 19), constituted 16-25% of the total. The fatty alcohols consisted of 14:0 (2%), 16:0 (66%), 18:0 (3%), 20:0 (8%), and 22:0 (21%). The sterols of Parauronema acutum consisted of cholesterol (53%), campesterol (32%), desmosterol (7%), and beta-sitosterol (8%).
对海洋纤毛虫急性副尾丝虫的中性脂质及其脂肪酸和甾醇组分进行了表征。中性脂质由甘油三酯(30%)、甾醇(29%)、游离脂肪酸(24%)、甾醇酯(9%)、甘油二酯(8%)和少量脂肪醇组成。尽管检测到了数量上的差异,但这些脂质的脂肪酸谱非常相似。饱和脂肪酸主要为14:0、16:0和18:0,占总量的20 - 30%。含有一至三个双键的不饱和脂肪酸主要为18:1(9)、18:2 (9,12)、18:3 (9, 12, 15)和20:3 (11, 14, 17),占总量的35 - 50%。高度不饱和脂肪酸18:4 (6, 9, 12, 15)、20:5 (5, 8, 11, 14, 17)和22:6 (4, 7, 10, 16, 19)占总量的16 - 25%。脂肪醇由14:0(2%)、16:0(66%)、18:0(3%)、20:0(8%)和22:0(21%)组成。急性副尾丝虫的甾醇由胆固醇(53%)、菜油甾醇(32%)、脱氢胆固醇(7%)和β-谷甾醇(8%)组成。