Curcio F, Ambesi-Impiombato F S, Perrella G, Coon H G
Istituto di Patologia Clinica e Sperimentale, Facoltà di Medicina, Universitá degli Studi di Udine, Italy.
Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9004-8. doi: 10.1073/pnas.91.19.9004.
We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expanded, cloned, frozen, successfully retrieved, and characterized. Functional characterization of these cells shows constitutive thyroglobulin production and release and thyrotropin-dependent adenosine 3',5'-cyclic monophosphate production, the latter apparently not associated with significant increases in DNA synthesis or cell proliferation. Genetic characterization of these cells by chromosome counting showed the normal diploid chromosome number. The ability to cultivate differentiated human thyroid follicular cells in long-term culture opens possibilities for investigating the transduction pathways of thyrotropin stimulation in normal and pathological human tissues, developing clinically relevant in vitro assays, and considering cellular and molecular therapies.
我们已经从正常成人甲状腺中获得了分化增殖性滤泡细胞的长期培养物。此类人类细胞的体外生长通过改良的F-12培养基得以维持，该培养基添加了牛下丘脑和垂体提取物，且未添加促甲状腺激素。培养物已得到扩增、克隆、冻存、成功复苏并进行了特性鉴定。这些细胞的功能特性表现为组成性甲状腺球蛋白的产生与释放以及促甲状腺激素依赖性3',5'-环磷酸腺苷的产生，后者显然与DNA合成或细胞增殖的显著增加无关。通过染色体计数对这些细胞进行的遗传学特性鉴定显示其染色体数目为正常二倍体。在长期培养中培养分化的人类甲状腺滤泡细胞的能力为研究正常和病理人类组织中促甲状腺激素刺激的转导途径、开发临床相关的体外检测方法以及考虑细胞和分子疗法开辟了可能性。
