Vérin P, Mortemousque B, Coulon P, Williamson W, Gendre P
Service d'Ophtalmologie, Centre J. Abadie, C.H.U. Bordeaux.
Rev Int Trach Pathol Ocul Trop Subtrop Sante Publique. 1993;70:179-83.
Immunofluorescence detection of C. trachomatis requires trained personnel because the threshold between positive and negative reaction is not obvious. Patients antibodies must cover EB and thus prevent binding with Mab. This problems have stimulated research on Chlamydiae probes DNA hybridization technology was applied and the specificity of the detection of CT was improved. Moreover, a polymerase chain reaction amplification of a fragment of about 1,200 bp of the M.O.M.P. gene followed by restriction endonuclease digestion with selected enzymes allows differentiation of serovars. This method seems to be promising for epidemiologic studies.
沙眼衣原体的免疫荧光检测需要训练有素的人员，因为阳性和阴性反应之间的界限不明显。患者抗体必须覆盖EB，从而阻止其与单克隆抗体结合。这些问题推动了对衣原体探针的研究，应用了DNA杂交技术，提高了沙眼衣原体检测的特异性。此外，对主要外膜蛋白（M.O.M.P.）基因约1200bp片段进行聚合酶链反应扩增，随后用选定的酶进行限制性内切酶消化，可区分血清型。这种方法在流行病学研究中似乎很有前景。
