通过基因扩增（聚合酶链反应或PCR）诊断眼部衣原体感染

[Diagnosis of ocular chlamydiosis by gene amplification (polymerase chain reaction or P.C.R.)].

作者信息

Vérin P, Gendre P, Goldschmidt P, Mortemousque B, Coulon P, Williamson W, Ndiaye P A

机构信息

C.H.R., Hôpital des Enfants, Service d'Ophtalmologie, Bordeaux.

出版信息

Rev Int Trach Pathol Ocul Trop Subtrop Sante Publique. 1992;69:133-7.

PMID:1344966

Abstract

Identification of conjunctival chlamydioses could be very difficult since this disease is extremely latent. Moreover previous instillation of topical antibiotic provokes a negativation of D.I.F. and of cultures. P.C.R. is very useful, it was demonstrative in 41 cases out of 47 cases; D.I.F. was positive only in 18 cases. P.C.R. is a very promising method to recognize chlamydiae; identification of different serovars is processing.

摘要

结膜衣原体病的诊断可能非常困难，因为这种疾病极具潜伏性。此外，先前局部使用抗生素会导致直接免疫荧光法（D.I.F.）检测结果及培养结果呈阴性。聚合酶链反应（P.C.R.）非常有用，在47例病例中有41例通过该方法得到确诊；直接免疫荧光法仅在18例病例中呈阳性。聚合酶链反应是一种极具前景的衣原体识别方法；不同血清型的鉴定正在进行中。