Jorgensen W K, Waldron S J
Tick Fever Research Centre, Queensland Department of Primary Industries, Wacol, Australia.
Vet Parasitol. 1994 May;53(1-2):45-51. doi: 10.1016/0304-4017(94)90015-9.
On nine separate occasions, Babesia bovis microaerophilus stationary phase (MASP) cultures were initiated with blood from calves with concurrent infections of B. bovis and Theileria buffeli, Eperythrozoon wenyoni or Anaplasma spp. In each case B. bovis became established in culture and was maintained for 30-49 days. Culture material was inoculated into susceptible splenectomised calves to test for persistence of the other organisms. No haemoparasites other than B. bovis were detected in Giemsa-stained blood films of recipient calves and no antibodies to T. buffeli or Anaplasma were detected using indirect fluorescent antibody and card agglutination tests, respectively. The method provides a practical way of removing contaminants such as Theileria, Eperythrozoon and Anaplasma spp. from B. bovis isolates without the use of drugs, tick passage or repeated syringe passage in cattle.
在九个不同的场合，用同时感染牛巴贝斯虫和水牛泰勒虫、温氏附红细胞体或无形体属的小牛血液启动牛巴贝斯虫嗜微氧静止期（MASP）培养物。在每种情况下，牛巴贝斯虫都能在培养物中建立并维持30 - 49天。将培养材料接种到易感的脾切除小牛中，以检测其他生物体的持续性。在受体小牛的吉姆萨染色血片中未检测到除牛巴贝斯虫以外的血液寄生虫，并且分别使用间接荧光抗体试验和卡片凝集试验未检测到针对水牛泰勒虫或无形体属的抗体。该方法提供了一种在不使用药物、蜱传代或在牛中反复注射器传代的情况下，从牛巴贝斯虫分离物中去除诸如泰勒虫、附红细胞体和无形体属等污染物的实用方法。
