通过聚合酶链反应检测梅毒患者脑脊液中的梅毒螺旋体。
Detection of Treponema pallidum by polymerase chain reaction in the cerebrospinal fluid of syphilis patients.
作者信息
Chung K Y, Lee M G, Lee J B
机构信息
Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Yonsei Med J. 1994 Jun;35(2):190-7. doi: 10.3349/ymj.1994.35.2.190.
To find out if polymerase chain reaction could be used as a diagnostic tool for detecting neurosyphilis, we have applied the PCR for the detection of Treponema pallidum DNA in the cerebrospinal fluid (CSF) of syphilis patients. The results of the PCR of the CSF in 26 patients with at various stages of illness were compared with the results of other conventional tests used in the WHO criteria. T. pallidum was detected in the CSF of patients at all stages of syphilis, which indicates that they invade the central nervous system from the early stages of infection. However, the presence of T. pallidum in the CSF was not correlated with the results of other tests used in the WHO criteria, and its significance in the diagnosis of neurosyphilis should further be evaluated.
为了确定聚合酶链反应是否可作为检测神经梅毒的诊断工具，我们应用聚合酶链反应检测梅毒患者脑脊液中的梅毒螺旋体DNA。将26例处于不同疾病阶段患者的脑脊液聚合酶链反应结果与世界卫生组织标准中使用的其他传统检测结果进行了比较。在梅毒各阶段患者的脑脊液中均检测到梅毒螺旋体，这表明它们从感染早期就侵入中枢神经系统。然而，脑脊液中梅毒螺旋体的存在与世界卫生组织标准中使用的其他检测结果无关，其在神经梅毒诊断中的意义应进一步评估。
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