各种处理和储存条件对脑脊液中梅毒螺旋体DNA稳定性的影响。
Effects of various handling and storage conditions on stability of Treponema pallidum DNA in cerebrospinal fluid.
作者信息
Villanueva A V, Podzorski R P, Reyes M P
机构信息
Department of Internal Medicine, Wayne State University, Detroit, Michigan 48201-1998, USA.
出版信息
J Clin Microbiol. 1998 Jul;36(7):2117-9. doi: 10.1128/JCM.36.7.2117-2119.1998.
Abstract
Treponema pallidum DNA from even small numbers of organisms was detectable in cerebrospinal fluid (CSF) stored at room temperature or at 4 degrees C for several hours and in CSF subjected to three freeze-thaw cycles. These results suggest that negative PCR results for T. pallidum from patients diagnosed with T. pallidum invasion of the central nervous system are probably not due to the loss of target DNA prior to testing.
摘要
即使是少量梅毒螺旋体的DNA，在室温或4℃下储存数小时的脑脊液中以及经过三次冻融循环的脑脊液中均可检测到。这些结果表明，被诊断为梅毒螺旋体侵犯中枢神经系统的患者梅毒螺旋体PCR检测结果为阴性，可能并非由于检测前靶DNA丢失所致。
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