Schröder H C, Lauc G, Sève A P, Hubert J, Flögel-Mrsic M, Müller W E
Abteilung für Angewandte Molekularbiologie, Universität, Mainz, Germany.
Z Gerontol. 1994 May-Jun;27(3):200-3.
Three carbohydrate-binding proteins with relative molecular masses of 35, 67, and 70 kDa (CBP35, CBP67, and CBP70) have been described to be present in nuclei of mammalian cells, where they are associated with nuclear ribonucleoprotein (RNP) complexes. CBP35 consists of two domains, an N-terminal domain that is homologous to certain regions of proteins of the heterogeneous nuclear RNP complex, and a C-terminal domain that is homologous to beta-galactoside-specific lectins. CBP35 has been proposed, like the glucose-specific lectin, CBP67, to guide RNP complexes through the nuclear pore. Here, we show that exposition of mature rats (6-8 months old) to stress results in binding of nuclear CBP35 to CBP67 which is retained on a column containing immobilized glucose. In contrast to mature animals, nuclear extracts from the livers of old rats (22-24 months old) displayed no detectable stress response.
已发现三种相对分子质量分别为35 kDa、67 kDa和70 kDa的碳水化合物结合蛋白（CBP35、CBP67和CBP70）存在于哺乳动物细胞的细胞核中，它们与核糖核蛋白（RNP）复合体相关。CBP35由两个结构域组成，一个N端结构域与不均一核RNP复合体蛋白质的某些区域同源，一个C端结构域与β-半乳糖苷特异性凝集素同源。有人提出，与葡萄糖特异性凝集素CBP67一样，CBP35可引导RNP复合体通过核孔。在此，我们表明，将成熟大鼠（6 - 8月龄）暴露于应激环境会导致细胞核中的CBP35与CBP67结合，这种结合物会保留在装有固定化葡萄糖的柱上。与成熟动物不同，老龄大鼠（22 - 24月龄）肝脏的核提取物未显示出可检测到的应激反应。
