Martin R, al-Sheibani A H, Gottschalk J, Martin H, Rotzsch W
Institut für Klinische Chemie und Pathobiochemie, Universität Leipzig.
Z Gerontol. 1994 May-Jun;27(3):194-9.
After physiological stress older male Wistar rats showed a faster normalization of the corticosterone level in serum and of the concentration of unoccupied glucocorticoid receptors in liver cytosol than young ones, which is contrary to female rats. The nuclei binding of glucocorticoid receptor complexes (GRC) was significantly reduced in older control animals compared with young ones. The nuclei binding of GRC was reduced in young rats until 60 min after stress compared to controls, whereas it had been increasing in older animals at this time. Contrary to female rats, the addition of the endogenous ATP-stimulated translocation promoter (ASTP) alone or together with ATP did not elevate the nuclei binding, but it groups. Crossing experiments using nuclei of controls and GRC of control and stressed animals, resp., seem to disclose a longer association of GRC with the nuclei in old animals than in young ones.
生理应激后，老年雄性Wistar大鼠血清中皮质酮水平和肝细胞溶质中未占据的糖皮质激素受体浓度比年轻大鼠恢复正常的速度更快，这与雌性大鼠相反。与年轻对照动物相比，老年对照动物中糖皮质激素受体复合物（GRC）的核结合显著减少。与对照组相比，年轻大鼠在应激后60分钟内GRC的核结合减少，而此时老年动物的GRC核结合一直在增加。与雌性大鼠相反，单独添加内源性ATP刺激转运启动子（ASTP）或与ATP一起添加均未提高核结合，但分组情况如此。分别使用对照细胞核以及对照和应激动物的GRC进行的交叉实验似乎表明，老年动物中GRC与细胞核的结合时间比年轻动物更长。
