Muylle S, Simoens P, Lauwers H
Department of Morphology, Faculty of Veterinary Medicine, University of Ghent, Belgium.
Zentralbl Veterinarmed A. 1994 Mar;41(2):143-9. doi: 10.1111/j.1439-0442.1994.tb00077.x.
The evolution of the healing process of venous puncture wounds was studied macroscopically and light-microscopically in order to determine the time of venipuncture. Seven small equids of various ages, all in normal healthy condition, were experimentally injected in the external jugular vein with a physiological Hartmann solution at different times before euthanasia. Two types of needle diameters i.e. 16.5 G and 19 G, were used. A fairly good uniformity in the healing process was seen in the 7 animals. In most cases the lesions induced within 5 weeks before death were macroscopically visible, whereas older perforation sites became indistinct. Light-microscopic study enabled determination of the time lapse after intravenous injection. The needle diameter seemed hereby relatively unimportant. Points of reference were endothelium repair, presence of a fibrin plug containing red and white blood cells, neovascularization and healing of the connective tissue fibers.
为了确定静脉穿刺时间，对静脉穿刺伤口的愈合过程进行了宏观和光学显微镜研究。在安乐死之前的不同时间，对7只不同年龄、健康状况正常的小型马科动物的颈外静脉进行了生理哈特曼溶液的实验性注射。使用了两种针径，即16.5G和19G。在这7只动物中观察到愈合过程具有相当好的一致性。在大多数情况下，死亡前5周内形成的损伤在宏观上可见，而较旧的穿孔部位则变得不明显。光学显微镜研究能够确定静脉注射后的时间间隔。由此看来，针径相对不重要。参考点包括内皮修复、含有红细胞和白细胞的纤维蛋白栓的存在、新血管形成以及结缔组织纤维的愈合。
