Burastero S E, Fenoglio D, Crimi E, Brusasco V, Rossi G A
DIBIT, San Raffaele Institute, Milan, Italy.
Allergy. 1994 May;49(5):348-53. doi: 10.1111/j.1398-9995.1994.tb02280.x.
We have recently shown that the number of peripheral allergen-specific T cells can, in part, predict, together with methacholine hyperresponsiveness, the bronchial response to inhaled allergen in asthmatic patients. This study was designed to explore the role of blood B cells committed to produce allergen-specific IgE in asthma. Twenty-three asthmatic patients sensitized to Dermatophagoides pteronyssinus and 11 control subjects were studied. Peripheral blood B cells, committed to produce allergen-specific IgE, were enumerated by limiting dilution microcultures of Epstein-Barr virus (EBV)-transformed B cells. An allergen inhalation challenge was performed in all asthmatic subjects. No difference was found in the frequency of B cells committed to produce allergen-specific IgE either between asthmatic patients and controls or between asthmatic patients with or without late-phase bronchial response to allergen. No correlation was found between the frequency of B cells committed to produce allergen-specific IgE and the bronchial response to the allergen inhalation challenge. We conclude that, in quantitative terms, peripheral allergen-specific B cells are not as relevant as T cells to the development of the asthmatic response in the model of provoked asthma.
我们最近发现，外周过敏原特异性T细胞的数量在一定程度上可以与乙酰甲胆碱高反应性一起预测哮喘患者对吸入性过敏原的支气管反应。本研究旨在探讨致力于产生过敏原特异性IgE的血液B细胞在哮喘中的作用。研究了23名对尘螨过敏的哮喘患者和11名对照受试者。通过对爱泼斯坦-巴尔病毒（EBV）转化的B细胞进行有限稀释微培养，对致力于产生过敏原特异性IgE的外周血B细胞进行计数。对所有哮喘受试者进行了过敏原吸入激发试验。在致力于产生过敏原特异性IgE的B细胞频率方面，哮喘患者与对照组之间，以及有或没有对过敏原迟发性支气管反应的哮喘患者之间均未发现差异。致力于产生过敏原特异性IgE的B细胞频率与过敏原吸入激发试验的支气管反应之间未发现相关性。我们得出结论，从数量上来说，在激发性哮喘模型中，外周过敏原特异性B细胞对哮喘反应发展的相关性不如T细胞。
