子宫内膜癌中甾体激素受体含量与流式细胞术DNA倍体的相关性

Association of steroid hormone receptor content and flow cytometric DNA ploidy in endometrial carcinoma.

作者信息

Grénman S E, Klemi P, Toikkanen S, Irjala K, Laippala P, Vähä-Eskeli K, Mäenpää J, Salmi T

机构信息

Department of Obstetrics and Gynaecology, Turku University Central Hospital, Finland.

出版信息

Ann Chir Gynaecol Suppl. 1994;208:10-4.

PMID:8092757

Abstract

Flow cytometric DNA ploidy and sex steroid hormone receptor activity was measured in 55 endometrial carcinomas. DNA ploidy was measured on paraffin-embedded specimens and ER and PR activity was measured using both biochemical and immunohistochemical assay. Association of these parameters was tested using logistic regression model. Diploid or near diploid DNA histogram was seen in 40 cases (72.7%). All Grade 1 tumours (n = 15) were diploid whereas 38.5% of Grade 2 (n = 27) and Grade 3 (n = 13) tumours were aneuploid (grade 1 vs Grade 2 and 3, P = 0.0031). The mean value of ER was 109 +/- 120 (SD) fmol/mg protein for diploid tumours and 48 +/- 50 fmol/mg protein for aneuploid tumours (P = 0.0093). The corresponding values for PR were 226 +/- 262 fmol/mg protein and 75 +/- 102 fmol/mg protein (P = 0.0073), respectively. In immunohistochemical assay the mean HSCORE of ER was 45 +/- 77 for diploid tumours and 27 +/- 48 for aneuploid tumours (P = 0.43). The corresponding values for PR were 32 +/- 55 and 22 +/- 56, respectively (P = 0.58). In logistic regression analysis cytosolic PR content was associated with DNA ploidy.

摘要

对55例子宫内膜癌进行了流式细胞术DNA倍体和性类固醇激素受体活性检测。在石蜡包埋标本上检测DNA倍体，采用生化和免疫组化方法检测雌激素受体（ER）和孕激素受体（PR）活性。使用逻辑回归模型检验这些参数之间的关联。40例（72.7%）可见二倍体或近二倍体DNA直方图。所有1级肿瘤（n = 15）均为二倍体，而2级（n = 27）和3级（n = 13）肿瘤中38.5%为非整倍体（1级与2级和3级相比，P = 0.0031）。二倍体肿瘤的ER平均值为109±120（标准差）fmol/mg蛋白，非整倍体肿瘤为48±50 fmol/mg蛋白（P = 0.0093）。PR的相应值分别为226±262 fmol/mg蛋白和75±102 fmol/mg蛋白（P = 0.0073）。在免疫组化检测中，二倍体肿瘤的ER平均HSCORE为45±77，非整倍体肿瘤为27±48（P = 0.43）。PR的相应值分别为32±55和22±56（P = 0.58）。在逻辑回归分析中，胞质PR含量与DNA倍体相关。