卵巢癌中的类固醇激素受体与流式细胞术DNA倍体分析
Steroid hormone receptors and flow cytometric DNA ploidy in ovarian carcinoma.
作者信息
Grénman S E, Klemi P, Toikkanen S, Kaihola H L, Laippala P, Mäenpää J, Mäkinen J, Grönroos M
机构信息
Department of Obstetrics and Gynaecology, Turku University Central Hospital, Finland.
出版信息
Ann Chir Gynaecol Suppl. 1994;208:15-9.
The biochemical and immunohistochemical estrogen (ER) and progesterone receptor (PR) content and flow cytometric DNA ploidy was analyzed in five semimalignant and 35 malignant epithelial ovarian tumours. By biochemical assay, 67% of the tumours were ER-positive (> or = 5 fmol/mg protein) and 56% were PR-positive (> or = 10 fmol/mg protein). The corresponding values by immunohistochemical assay (with a HSCORE of 10 as the cutoff level) were 22% and 27%, respectively. DNA histogram measured from paraffin embedded specimens were diploid in 20% (7/35) and aneuploid in 80% (28/35) of the malignant tumours. All semimalignant tumours were diploid. The mean receptor values in the diploid and aneuploid tumours did not differ significantly and receptor-positive and receptor-negative tumours were evenly distributed in all stages and grades. In contrast, flow cytometric DNA ploidy was clearly associated with tumour stage (G2 = 10.52, Df = 3, P = 0.015) and histological differentiation (G2 = 20.57, Df = 3, P = 0.0001).
对5例半恶性和35例恶性上皮性卵巢肿瘤进行了生化、免疫组化雌激素（ER）和孕激素受体（PR）含量分析以及流式细胞术DNA倍体分析。通过生化检测，67%的肿瘤ER阳性（≥5 fmol/mg蛋白），56%的肿瘤PR阳性（≥10 fmol/mg蛋白）。免疫组化检测（以HSCORE为10作为截断水平）的相应值分别为22%和27%。从石蜡包埋标本测量的DNA直方图显示，20%（7/35）的恶性肿瘤为二倍体，80%（28/35）为非整倍体。所有半恶性肿瘤均为二倍体。二倍体和非整倍体肿瘤的平均受体值无显著差异，受体阳性和受体阴性肿瘤在所有分期和分级中分布均匀。相比之下，流式细胞术DNA倍体与肿瘤分期（G2 = 10.52，自由度 = 3，P = 0.015）和组织学分化（G2 = 20.57，自由度 = 3，P = 0.0001）明显相关。
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