Clark I M, Powell L K, Cawston T E
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):874-80. doi: 10.1006/bbrc.1994.2264.
This study aimed to investigate possible feedback mechanisms in the control of collagenase (matrix metalloproteinase-1) and tissue inhibitor of metalloproteinases (TIMP-1). Procollagenase, active collagenase, TIMP-1 and collagenase-TIMP complex were applied to human skin and synovial fibroblasts for 48 hours, the cells were washed and the resulting collagenase and TIMP-1 secretion was measured over the following 72 hours using ELISAs. Of the additions, only TIMP-1 showed any measurable effect, stimulating collagenase secretion over a range of 1-8 micrograms/ml. Endotoxin contamination was ruled out as an explanation and the active conformation of the TIMP-1 molecule was shown to be necessary.
本研究旨在探究胶原酶（基质金属蛋白酶-1）和金属蛋白酶组织抑制剂（TIMP-1）调控过程中可能存在的反馈机制。将前胶原酶、活性胶原酶、TIMP-1和胶原酶-TIMP复合物作用于人类皮肤和滑膜成纤维细胞48小时，清洗细胞后，在接下来的72小时内使用酶联免疫吸附测定法（ELISA）测量产生的胶原酶和TIMP-1分泌量。在所添加的物质中，只有TIMP-1显示出任何可测量的效应，在1-8微克/毫升的范围内刺激胶原酶分泌。排除了内毒素污染作为一种解释，并且表明TIMP-1分子的活性构象是必需的。
