Akimoto S, Ono T, Tsutsui H, Kinouchi T, Kataoka K, Ohnishi Y
Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):914-21. doi: 10.1006/bbrc.1994.2269.
The nucleotide sequence of the Bacteroides fragilis neuraminidase-encoding gene (nanH) and its flanking regions was determined. The B. fragilis nanH open reading frame (ORF) of 1632 nucleotides encoded a neuroaminidase of 544 amino acid residues with a calculated molecular mass of 59.51 kDa. The amino acid sequence at the N-terminus of the deduced protein exhibited the common features of procaryotic signal sequences. The Asp box sequence, Ser-X-Asp-X-Gly-X-Thr-Trp, which is conserved among bacterial neuraminidases, was found at five positions in the deduced amino acid sequence. A single transcription start point was identified 88 nucleotides upstream of the nanH ORF. Northern blot analysis using an internal nanH probe revealed a single mRNA species with a length of 1.8 kb that could encode just the neuraminidase.
测定了脆弱拟杆菌神经氨酸酶编码基因（nanH）及其侧翼区域的核苷酸序列。脆弱拟杆菌nanH开放阅读框（ORF）由1632个核苷酸组成，编码一个含有544个氨基酸残基的神经氨酸酶，计算分子量为59.51 kDa。推导蛋白N端的氨基酸序列具有原核信号序列的共同特征。在推导的氨基酸序列中的五个位置发现了细菌神经氨酸酶中保守的Asp框序列Ser-X-Asp-X-Gly-X-Thr-Trp。在nanH ORF上游88个核苷酸处鉴定出一个单一的转录起始点。使用nanH内部探针的Northern印迹分析显示有一个长度为1.8 kb的单一mRNA种类，其仅能编码神经氨酸酶。
