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从中国分离出的两株斑点热群立克次体新菌株的基因型和抗原鉴定

Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China.

作者信息

Yu X, Jin Y, Fan M, Xu G, Liu Q, Raoult D

机构信息

Unité des Rickettsies, Faculté de Médecine, Marseille, France.

出版信息

J Clin Microbiol. 1993 Jan;31(1):83-8. doi: 10.1128/jcm.31.1.83-88.1993.

DOI:10.1128/jcm.31.1.83-88.1993
PMID:8093253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262626/
Abstract

Four isolates of spotted fever group rickettsiae isolated from ticks in China were compared with all known species and strains of spotted fever group rickettsiae by immunofluorescence assay, DNA polymerase chain reaction followed by restriction endonuclease fragment length polymorphism analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblot. The Chinese isolates belonged to three types, including a novel serotype which has not been described before. One isolate obtained from tick ova of Dermacentor nuttallii in Inner Mongolia was antigenically and genotypically identical to Rickettsia sibirica. Two isolates obtained from Dermacentor sinicus collected from Beijing were identical, different from other members of spotted fever group rickettsiae but apparently closely related to R. sibirica. HA-91, a strain isolated from Hyalomma asiaticum bv. kozlovi olenew, was antigenically and genotypically unique among spotted fever group rickettsiae, and we feel that data presented here should prompt consideration of it as a new species on the basis of current rickettsial taxonomy.

摘要

通过免疫荧光试验、DNA聚合酶链反应及限制性内切酶片段长度多态性分析、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和Western免疫印迹，将从中国蜱中分离出的4株斑点热群立克次体与斑点热群立克次体的所有已知种和菌株进行了比较。中国分离株属于三种类型，包括一种以前未描述过的新血清型。从内蒙古草原革蜱蜱卵中获得的一株分离株在抗原性和基因型上与西伯利亚立克次体相同。从北京采获的中华革蜱中获得的两株分离株相同，与斑点热群立克次体的其他成员不同，但显然与西伯利亚立克次体密切相关。从亚洲璃眼蜱蒙古亚种olenew株分离出的HA-91菌株在抗原性和基因型上在斑点热群立克次体中是独特的，我们认为此处提供的数据应促使根据当前立克次体分类法将其视为一个新种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/d5759091c0d7/jcm00013-0105-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/3a131593507e/jcm00013-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/90abc4689f4f/jcm00013-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/c48ed66badca/jcm00013-0103-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/4b37d4ecd1c8/jcm00013-0103-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/9077570f4800/jcm00013-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/752bf10e78bf/jcm00013-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/129fc18417ef/jcm00013-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/d5759091c0d7/jcm00013-0105-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/3a131593507e/jcm00013-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/90abc4689f4f/jcm00013-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/c48ed66badca/jcm00013-0103-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/4b37d4ecd1c8/jcm00013-0103-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/9077570f4800/jcm00013-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/752bf10e78bf/jcm00013-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/129fc18417ef/jcm00013-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9728/262626/d5759091c0d7/jcm00013-0105-b.jpg

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