Romani L, Mencacci A, Cenci E, Spaccapelo R, Mosci P, Puccetti P, Bistoni F
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
J Immunol. 1993 Feb 1;150(3):925-31.
Previous work has shown that in hybrid (BALB/cCr x DBA/2Cr)F1 mice the development of a fatal disseminated disease by systemic infection with virulent Candida albicans is associated with the detection of strong Th2-like responses. However, a predominant Th1-like response and long-lived antifungal protection are induced by vaccinating these mice with live blastospores of attenuated C. albicans strains. When injected into DBA/2Cr mice, one such live vaccine strain was found in the present study to result in a progressive disease characterized by strong Th2 responses. Elevated serum IgG1, IgA, and IgE responses, weak or absent footpad reactions, sustained production in vitro of Th2 (IL-4 and IL-10) but not Th1 (IL-2 and IFN-gamma) cytokines by CD4+ cells, and eosinophilia were all detected in DBA/2 mice after infection with the attenuated vaccine. This was in marked contrast with the development of strong Th1 responses and persistent anticandidal protection in similarly infected, H-2-compatible BALB/cCr mice. Therefore, our data suggest that the type of Th response that predominates in mice after C. albicans infection correlates with genetically determined susceptibility or vaccine-induced resistance. Moreover, the genetic control of this resistance may not be associated with the H-2 complex.
先前的研究表明，在（BALB/cCr×DBA/2Cr）F1杂交小鼠中，用致病性白色念珠菌进行全身感染导致致命性播散性疾病的发展与检测到强烈的Th2样反应有关。然而，用减毒白色念珠菌菌株的活芽生孢子对这些小鼠进行疫苗接种可诱导主要的Th1样反应和长期的抗真菌保护。在本研究中发现，当将一种这样的活疫苗菌株注射到DBA/2Cr小鼠中时，会导致以强烈Th2反应为特征的进行性疾病。在感染减毒疫苗后的DBA/2小鼠中，检测到血清IgG1、IgA和IgE反应升高、足垫反应微弱或缺失、CD4+细胞在体外持续产生Th2（IL-4和IL-10）而非Th1（IL-2和IFN-γ）细胞因子以及嗜酸性粒细胞增多。这与在同样感染、H-2相容的BALB/cCr小鼠中强烈Th1反应的发展和持续的抗念珠菌保护形成明显对比。因此，我们的数据表明，白色念珠菌感染后在小鼠中占主导的Th反应类型与遗传决定的易感性或疫苗诱导的抗性相关。此外，这种抗性的遗传控制可能与H-2复合体无关。
