Hausner M A, Giorgi J V, Plaeger-Marshall S
Department of Medicine, University of California, School of Medicine, Los Angeles.
J Immunol Methods. 1993 Jan 4;157(1-2):181-7. doi: 10.1016/0022-1759(93)90085-l.
CD8 T lymphocytes are an important component of the host immune response to human immunodeficiency virus (HIV). To characterize CD8 cell function, we have studied the in vitro phenomenon of CD8 cell-mediated inhibition of HIV replication from autologous, naturally infected CD4 cells. We describe here a reproducible assay of CD8 T cell-mediated inhibition in HIV-infected individuals. The method involves the use of a commercially available cell separation system and anti-CD3 monoclonal antibody to stimulate CD4 cells to produce HIV. Using this technique, we were able to detect HIV production from the CD4 cells of 25 of 27 HIV-infected individuals who had not progressed to AIDS. Further, in vitro CD8 cell-mediated inhibition of HIV production was noted in all of the 25 subjects whose CD4 cells produced viral p24 antigen. This assay may be useful as an in vitro correlate of protective immunity to HIV, with potential application for assessing disease progression, therapeutic efficacy, and immune mechanisms in HIV disease.
CD8 T淋巴细胞是宿主对人类免疫缺陷病毒（HIV）免疫反应的重要组成部分。为了表征CD8细胞的功能，我们研究了CD8细胞介导的对来自自体自然感染的CD4细胞的HIV复制进行抑制的体外现象。我们在此描述一种在HIV感染个体中可重复的CD8 T细胞介导抑制的检测方法。该方法涉及使用市售的细胞分离系统和抗CD3单克隆抗体来刺激CD4细胞产生HIV。使用这种技术，我们能够在27名尚未发展为艾滋病的HIV感染个体中的25名个体的CD4细胞中检测到HIV的产生。此外，在其CD4细胞产生病毒p24抗原的所有25名受试者中均观察到体外CD8细胞介导的HIV产生抑制作用。该检测方法可能作为HIV保护性免疫的体外相关指标有用，具有评估HIV疾病进展、治疗效果和免疫机制的潜在应用价值。
