Chen Y, Rinaldo C, Gupta P
Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health and School of Medicine, Pennsylvania 15261, USA.
Clin Diagn Lab Immunol. 1997 Jan;4(1):4-10. doi: 10.1128/cdli.4.1.4-10.1997.
A reproducible, semiquantitative assay was developed to measure the level of the anti-human immunodeficiency virus type 1 (anti-HIV-1) suppressive activity of CD8+ T cells. The assay had a wide dynamic range and could be applied to a relatively small number of fresh and cryopreserved peripheral blood mononuclear cells and purified CD8+ T cells. The suppressive activity was not due to cytolytic activity and was not major histocompatibility complex class I restricted. Suppression of HIV-1 infection by CD8+ T cells was consistently demonstrable with both endogenously infected autologous CD4+ T cells and exogenously infected allogeneic CD4+ T cells. This assay can be used to monitor the level of antiviral activity of CD8+ T cells in a retrospective and prospective manner in studies of the natural history of HIV-1 infection and of subjects receiving anti-HIV-1 therapy and vaccines.
开发了一种可重复的半定量检测方法，用于测量CD8 + T细胞的抗人免疫缺陷病毒1型（抗HIV-1）抑制活性水平。该检测方法具有较宽的动态范围，可应用于相对少量的新鲜和冷冻保存的外周血单个核细胞以及纯化的CD8 + T细胞。抑制活性不是由于细胞溶解活性，并且不受主要组织相容性复合体I类限制。用内源性感染的自体CD4 + T细胞和外源性感染的同种异体CD4 + T细胞均能持续证明CD8 + T细胞对HIV-1感染的抑制作用。该检测方法可用于回顾性和前瞻性监测HIV-1感染自然史研究以及接受抗HIV-1治疗和疫苗的受试者中CD8 + T细胞的抗病毒活性水平。
