Kottel R H, Bacon K, Clutter D, White D
J Bacteriol. 1975 Oct;124(1):550-7. doi: 10.1128/jb.124.1.550-557.1975.
An extracellular coat from glycerol-induced myxospores of Myxococcus xanthus has been isolated and characterized. Coats were examined chemically and by using both transmission and scanning electron microscopy. On a dry weight basis, approximately 75% of the coat is polysaccharide composed entirely of galactosamine and glucose. The remainder of the coat is protein (14%), glycine (8%), and organic phosphorus (less than 1%). Coats remained morphologically intact despite boiling in 10 M urea, sodium lauryl sulfate plus beta-mercaptoethanol, or extraction with warm phenol. Coats also resisted digestion with a variety of proteolytic and polysaccharide degrading enzymes. Synthesis of myxospore coat begins approximately 1 h after the addition of glycerol to a culture. One portion of the coat is complete by 5 to 6 h but additional material consisting primarily of glucose is added after 8 h.
已分离并鉴定了来自黄色粘球菌甘油诱导的粘孢子的细胞外被膜。通过化学方法以及透射电子显微镜和扫描电子显微镜对被膜进行了检查。以干重计，约75%的被膜是完全由半乳糖胺和葡萄糖组成的多糖。被膜的其余部分是蛋白质（14%）、甘氨酸（8%）和有机磷（少于1%）。尽管在10 M尿素、十二烷基硫酸钠加β-巯基乙醇中煮沸或用温苯酚提取，被膜在形态上仍保持完整。被膜也能抵抗多种蛋白水解酶和多糖降解酶的消化。向培养物中添加甘油后约1小时开始合成粘孢子被膜。被膜的一部分在5至6小时内完成，但主要由葡萄糖组成的额外物质在8小时后添加。
