Spadinger I, Palcic B
BC Cancer Research Centre, Vancouver, Canada.
Int J Radiat Biol. 1993 Feb;63(2):183-9. doi: 10.1080/09553009314550241.
The statistical precision of cell survival data measured at low doses can be greatly improved if the number of cells assayed at each dose point is known exactly. This paper describes an image cytometry technique that has been developed for this purpose. Treated cells are plated into tissue culture flasks, and after attachment their locations are determined with 98% accuracy by automated scanning procedures. These locations are revisited after an appropriate incubation period to assay for colony formation. Colonies may be scored either manually or using image data collected by the device. Additional information, such as heterogeneity of colony size, can also be obtained.
如果能确切知道每个剂量点所检测的细胞数量，那么在低剂量下测量的细胞存活数据的统计精度可得到极大提高。本文描述了一种为此目的而开发的图像细胞术技术。将处理后的细胞接种到组织培养瓶中，贴壁后通过自动扫描程序以98%的准确率确定其位置。在适当的孵育期后再次查看这些位置，以检测集落形成情况。集落可以手动计数，也可以使用该设备收集的图像数据进行计数。还可以获得其他信息，例如集落大小的异质性。
