Chahal R, Craig D Q, Pinney R J
Microbiology Section, School of Pharmacy, University of London, UK.
J Pharm Pharmacol. 1993 Jan;45(1):30-3. doi: 10.1111/j.2042-7158.1993.tb03674.x.
Exposure of growing cells of Escherichia coli strain AB1157 to a frequency of 1 Hz with field strengths of 1 or 3 kV m-1 did not affect spontaneous or ultraviolet light (UV)-induced mutation frequencies to rifampicin resistance. Neither did growth in the presence of charge alter the sensitivities of strains AB1157, TK702 umuC or TK501 umuC uvrB to UV. Similarly, although the resistance of strains TK702 umuC and TK501 umuC uvrB to UV was increased by the presence of plasmid pKM101, which carries DNA repair genes, pregrowth of plasmid-containing strains in electric fields did not increase UV resistance. Finally, growth in a low frequency field in the presence of sub-inhibitory concentrations of mitomycin C did not affect mitomycin C-induced mutation frequencies. It is concluded that low frequency electromagnetic fields do not increase spontaneous mutation, induce DNA repair or increase the mutagenic effects of UV or mitomycin C.
将大肠杆菌AB1157菌株的生长细胞暴露于频率为1 Hz、场强为1或3 kV m-1的环境中，不会影响对利福平耐药性的自发或紫外线（UV）诱导的突变频率。在有电荷存在的情况下生长也不会改变AB1157、TK702 umuC或TK501 umuC uvrB菌株对紫外线的敏感性。同样，尽管携带DNA修复基因的质粒pKM101的存在增加了TK702 umuC和TK501 umuC uvrB菌株对紫外线的抗性，但含质粒菌株在电场中的预生长并未增加其对紫外线的抗性。最后，在亚抑制浓度的丝裂霉素C存在下于低频场中生长，不会影响丝裂霉素C诱导的突变频率。得出的结论是，低频电磁场不会增加自发突变、诱导DNA修复或增加紫外线或丝裂霉素C的诱变作用。
