Kumamoto K, Ebara S, Matsuura T
Department of Anatomy, Meiji College of Oriental Medicine, Kyoto, Japan.
Acta Anat (Basel). 1993;146(1):46-52. doi: 10.1159/000147420.
Pacinian corpuscles in the cat urinary bladder were studied using the hematoxylin and eosin staining, formaldehyde-induced fluorescence histochemistry, immunohistochemistry for tyrosine hydroxylase (TH) and immunoelectron microscopy. The existence of catecholaminergic nerve fibers penetrating into the corpuscles was clarified. (1) About 10 Pacinian corpuscles which were mainly located in the subserosa were found in the lower one-third portion of each urinary bladder. (2) One or two corpuscles containing blue-green fluorescent fibers were detected in each bladder. These fibers were equivalent to TH-immunopositive varicose fibers using the avidin-biotin-peroxidase complex method. (3) Thin nerve fibers containing dense-cored vesicles were found in the corpuscle by immunoelectron microscopy. These fibers spirally encompassed a swollen sensory terminal at a distance in the inner core. According to the above findings, we discuss a modulatory function of the catecholaminergic fibers in this mechanoreceptor.
利用苏木精-伊红染色、甲醛诱导荧光组织化学、酪氨酸羟化酶（TH）免疫组织化学及免疫电镜技术，对猫膀胱中的帕西尼小体进行了研究。明确了有儿茶酚胺能神经纤维穿入这些小体。（1）在每个膀胱下三分之一部分发现约10个主要位于浆膜下的帕西尼小体。（2）在每个膀胱中检测到一两个含有蓝绿色荧光纤维的小体。使用抗生物素蛋白-生物素-过氧化物酶复合物法，这些纤维等同于TH免疫阳性的曲张纤维。（3）通过免疫电镜在小体中发现了含有致密核心小泡 的细神经纤维。这些纤维在内核中以一定距离螺旋状环绕着一个肿胀的感觉终末。根据上述发现，我们讨论了儿茶酚胺能纤维在这种机械感受器中的调节功能。
