Picollo M I, Seccacini E, Vassena C, Zerba E
Centro de Investigaciones de Plagas e Insecticidas-CIPEIN-(CIEFA-CONICET, Pcia. de Buenos Aires, Argentina.
Acta Trop. 1993 Jan;52(4):297-307. doi: 10.1016/0001-706x(93)90014-3.
Topical application of different sulfhydryl (SH) reagents in acetone solution resulted in a deterrence of the feeding activity of Triatoma infestans nymph V. The ED50 for topical application of N-ethylmaleimide (NEM), the more active compound assayed, was 8.9 micrograms/insect. This effect showed a dose-dependent reversibility. We also observed feeding deterrency when a gauze cloth impregnated with NEM was located between the food source and the nymphs. After topical NEM treatment, mating deterrency was assessed by counting spermatophores dropped and eggs laid. When a population of adult insects, nymphal stages and eggs was reared in a cage containing filter papers treated with 0.4 mg/cm2 of NEM, a significant decrease of population density was seen after 200 days of exposure. Weekly replacement of filter papers impregnated with 0.13 mg/cm2 NEM for a period of one year enabled population control to be maintained. Antifeeding and antimating effects of NEM in Triatoma infestans and the resulting population control could be attributed to a chemoreceptor blockage produced by SH reagents.
在丙酮溶液中局部应用不同的巯基（SH）试剂，可抑制骚扰锥蝽若虫V的取食活动。所检测的活性更强的化合物N - 乙基马来酰亚胺（NEM）局部应用的半数有效剂量（ED50）为8.9微克/昆虫。这种效应呈现剂量依赖性可逆性。当在食物源和若虫之间放置一块浸有NEM的纱布时，我们也观察到了取食抑制作用。局部应用NEM处理后，通过计数掉落的精包和产下的卵来评估交配抑制作用。当在一个装有经0.4毫克/平方厘米NEM处理的滤纸的笼子里饲养成虫、若虫阶段和卵的群体时，暴露200天后观察到种群密度显著下降。每周更换浸有0.13毫克/平方厘米NEM的滤纸，持续一年，可维持种群控制。NEM对骚扰锥蝽的拒食和抗交配作用以及由此产生的种群控制，可能归因于SH试剂导致的化学感受器阻滞。
