风疹沉淀原的大规模生产及其在诊断实验室中的应用。
Large-scale production of rubella precipitinogens and their use in the diagnostic laboratory.
作者信息
Cappel R, Schluederberg A, Horstmann D M
出版信息
J Clin Microbiol. 1975 Feb;1(2):201-5. doi: 10.1128/jcm.1.2.201-205.1975.
Abstract
A method for producing large quantities of rubella theta and iota precipitating antigens in Vero cells is described. Using this reagent, the detection of anti-theta antibodies was found to be as sensitive as the hemagglutination inhibition test for both the determination of immune status and the diagnosis of acute rubella infection. The detection of anti-iota antibodies may permit diagnosis of rubella infection, when the collection of the first serum has been delayed and the early rise of hemagglutin-inhibiting and anti-theta antibodies has been missed.
摘要
描述了一种在非洲绿猴肾细胞（Vero细胞）中大量生产风疹θ和ι沉淀抗原的方法。使用该试剂，发现检测抗θ抗体对于确定免疫状态和诊断急性风疹感染的敏感性与血凝抑制试验相当。当首次采集血清延迟且错过了血凝抑制抗体和抗θ抗体的早期升高时，检测抗ι抗体可能有助于诊断风疹感染。
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