Wigley W C, Corson G E, Nakashima R A
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409.
Int J Cancer. 1993 Feb 20;53(4):657-61. doi: 10.1002/ijc.2910530422.
We have compared genomic DNA isolated from a series of rat hepatomas and from normal rat liver tissue by restriction fragment analysis. Our objective was to identify a tumor-specific restriction fragment that would be present in rapidly growing tumors and missing in normal tissues. Using a cDNA probe specific for the C-terminal half of rat brain hexokinase, we have identified several changes in DNA restriction fragment lengths in rat hepatomas vs. normal rat liver. Most of these involved restriction fragments that were present in normal rat DNA and missing in tumor DNA. However, one HinfI fragment of M(r) = 13.4 kilobase pairs (kb) was found to be present in rapidly growing rat hepatomas, but missing in normal rat tissue and in hepatomas of slow or intermediate growth rate.
我们通过限制性片段分析比较了从一系列大鼠肝癌以及正常大鼠肝脏组织中分离出的基因组DNA。我们的目标是鉴定出一个肿瘤特异性限制性片段，该片段在快速生长的肿瘤中存在，而在正常组织中缺失。使用针对大鼠脑己糖激酶C端一半的cDNA探针，我们已鉴定出大鼠肝癌与正常大鼠肝脏中DNA限制性片段长度的若干变化。其中大多数涉及正常大鼠DNA中存在而肿瘤DNA中缺失的限制性片段。然而，发现一个分子量为13.4千碱基对（kb）的HinfI片段存在于快速生长的大鼠肝癌中，但在正常大鼠组织以及生长缓慢或中等的肝癌中缺失。
