Wigley W C, Nakashima R A
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409.
FEBS Lett. 1992 Mar 30;300(2):153-6. doi: 10.1016/0014-5793(92)80185-j.
We have compared Southern blots of rat hepatoma DNA probed with Types I, II and III hexokinase cDNAs isolated from normal rat tissues. Hybridization patterns show several fragments recognized by both the Type I and II clones while no resemblance is observed between the Type III probe and the other two isozymes. It therefore appears that the Type I-like and Type II-like hepatoma isozymes are coded for by similar yet separate genes, while a dissimilar third gene codes for the Type III-like isozyme. In addition, a loss of heterozygosity was detected at the Type III locus in the AS-30D hepatoma when compared to normal tissue. As only the Type II-like isozyme is highly expressed in highly glycolytic tumors, these data have implications for differential gene regulation between the tumor isozymes.
我们比较了用从正常大鼠组织中分离出的I型、II型和III型己糖激酶cDNA探测的大鼠肝癌DNA的Southern杂交结果。杂交模式显示I型和II型克隆都能识别几个片段，而III型探针与其他两种同工酶之间没有相似之处。因此，I型样和II型样肝癌同工酶似乎由相似但不同的基因编码，而不同的第三个基因编码III型样同工酶。此外，与正常组织相比，在AS - 30D肝癌的III型基因座处检测到杂合性缺失。由于只有II型样同工酶在高糖酵解肿瘤中高度表达，这些数据对肿瘤同工酶之间的差异基因调控具有启示意义。
