Kaur I, Voss S D, Gupta R S, Schell K, Fisch P, Sondel P M
Department of Human Oncology, University of Wisconsin, Madison 53792.
J Immunol. 1993 Mar 1;150(5):2046-55.
Studies with the use of polyclonal rabbit antiserum reactive with 60-kDa heat shock proteins (hsp60) suggested that hsp60-related molecules could be found on the surface of Daudi cells and were involved in their recognition by certain human gamma delta T cells. The present study confirms this finding by using a mAb specifically recognizing hsp60. This mAb can block outgrowth of human gamma delta T cells in response to stimulation with Daudi and in response to an extract of the mycobacteria H37Ra. This anti-hsp60 mAb stains the surface of Daudi cells, but does not stain either Raji or EBV-transformed B cells, cells which do not stimulate gamma delta T cell outgrowth. Anti-hsp60 mAb could immunoprecipitate a 60-kDa molecule from the H37Ra extract but was unable to precipitate this 60-kDa molecule if the mAb was first absorbed on Daudi cells. This mAb also precipitated a 60-kDa molecule from the surface of Daudi cells which shows an electrophoretic mobility pattern consistent with hsp60. These experiments demonstrate that human gamma delta T cells recognize hsp60-related epitopes on the surface of Daudi cells and within mycobacterial extracts.
使用与60 kDa热休克蛋白（hsp60）反应的兔多克隆抗血清进行的研究表明，hsp60相关分子可在Daudi细胞表面发现，并参与某些人γδT细胞对它们的识别。本研究通过使用特异性识别hsp60的单克隆抗体（mAb）证实了这一发现。该mAb可阻断人γδT细胞在受到Daudi刺激以及受到结核分枝杆菌H37Ra提取物刺激时的生长。这种抗hsp60 mAb可对Daudi细胞表面进行染色，但对Raji细胞或EBV转化的B细胞（即不刺激γδT细胞生长的细胞）均不染色。抗hsp60 mAb可从H37Ra提取物中免疫沉淀出一个60 kDa的分子，但如果该mAb首先吸附在Daudi细胞上，则无法沉淀出这个60 kDa的分子。该mAb还从Daudi细胞表面沉淀出一个60 kDa的分子，其电泳迁移模式与hsp60一致。这些实验表明，人γδT细胞可识别Daudi细胞表面以及分枝杆菌提取物中的hsp60相关表位。
