气相色谱/化学反应接口质谱法对含氯化合物的选择性检测

Selective detection of chlorine-containing compounds by gas chromatography/chemical reaction interface mass spectrometry.

作者信息

Song H, Abramson F P

机构信息

Department of Pharmacology, George Washington University, Washington, D.C. 20037.

出版信息

Anal Chem. 1993 Feb 15;65(4):447-50. doi: 10.1021/ac00052a022.

DOI:10.1021/ac00052a022

PMID:8094946

Abstract

Chemical reaction interface mass spectrometry (CRIMS) was studied as a gas chromatographic detection technique for chlorine-containing compounds. Both SO2 and HBr were tested as reactant gases. With SO2, a detection limit of 50 pg of diazepam and a linear range of 4 orders of magnitude were achieved, and the experimental data were reproducible. With HBr, the detection limit was 10 ng of diazepam and the linear dynamic range was only 2 orders of magnitude. The possible pharmacological application of CRIMS was studied using urine spiked with diazepam and several of its metabolites, and the results show CRIMS to be a simple but potentially powerful method in drug metabolism studies.

摘要

化学反应界面质谱法（CRIMS）作为一种用于含氯化合物的气相色谱检测技术进行了研究。二氧化硫（SO₂）和溴化氢（HBr）都被用作反应气体进行了测试。使用SO₂时，地西泮的检测限为50皮克，线性范围为4个数量级，并且实验数据具有可重复性。使用HBr时，地西泮的检测限为10纳克，线性动态范围仅为2个数量级。利用添加了地西泮及其几种代谢物的尿液研究了CRIMS在药理学方面的可能应用，结果表明CRIMS在药物代谢研究中是一种简单但可能很强大的方法。