Chau F, Levacher-Clergeot M, Desforges B, Ricatte L, Sinet M
U13 INSERM, Hôpital Bichat-Claude Bernard, Paris, France.
Clin Exp Immunol. 1993 Mar;91(3):467-72. doi: 10.1111/j.1365-2249.1993.tb05926.x.
Mice infected with the Duplan strain of murine leukaemia virus (Dup MuLV), a retrovirus, develop a syndrome sharing several features with AIDS, including lymphadenopathy and profound immunodeficiency. We measured the changes in peripheral blood lymphocyte populations and evaluated their predictive value for the outcome of disease in C57Bl/6 mice. Animals were inoculated with Dup MuLV (SC1/Dup MuLV confluent fibroblast supernatant or spleen extract from an infected mouse). Peripheral blood lymphocyte subsets were sequentially monitored for 73 days using flow cytometric analysis and MoAbs directly conjugated to fluorochromes. A striking fall in the Thy1.2+ cell count occurred in diseased animals, mostly affecting the CD8+ cell compartment. At the same time, the percentage of Ly5+ cells was increased. Mice were killed at day 73 and spleen and lymph node lymphocytes were analysed. Phenotypic lymphocyte modifications in peripheral blood were closely related to those in the spleen or lymph nodes. Analysis of Ly6c antigen expression on CD4+ and CD8+ cells showed a selective expansion of the CD8+Ly6c+ subset, which may reflect a state of immune activation. Our results suggest that phenotypic alterations of peripheral blood lymphocytes are a good marker of disease progression in this model and could be a useful criterion to evaluate antiretroviral therapy.
感染了鼠白血病病毒（Dup MuLV）杜普兰株（一种逆转录病毒）的小鼠会出现一种与艾滋病有若干共同特征的综合征，包括淋巴结病和严重免疫缺陷。我们检测了C57Bl/6小鼠外周血淋巴细胞群体的变化，并评估了它们对疾病预后的预测价值。动物接种Dup MuLV（SC1/Dup MuLV汇合的成纤维细胞上清液或来自感染小鼠的脾提取物）。使用直接与荧光染料偶联的单克隆抗体，通过流式细胞术分析连续73天监测外周血淋巴细胞亚群。患病动物的Thy1.2 +细胞计数显著下降，主要影响CD8 +细胞区室。同时，Ly5 +细胞的百分比增加。在第73天处死小鼠，并分析脾和淋巴结淋巴细胞。外周血中淋巴细胞的表型改变与脾或淋巴结中的表型改变密切相关。对CD4 +和CD8 +细胞上Ly6c抗原表达的分析显示CD8 + Ly6c +亚群选择性扩增，这可能反映了免疫激活状态。我们的结果表明，外周血淋巴细胞的表型改变是该模型中疾病进展的良好标志物，并且可能是评估抗逆转录病毒疗法的有用标准。
