Przybylska M, Bryszewska M, Chapman I V
Department of Biophysics, University of Lodz, Poland.
Int J Radiat Biol. 1993 Mar;63(3):419-24. doi: 10.1080/09553009314550551.
Exposure of human erythrocytes to elevated temperatures induces a decrease in stability of the cell membrane. Thermally induced haemolysis of erythrocytes from patients with type 1 diabetes and from healthy control individuals was measured as a function of duration of exposure to heat between 48.0 and 54.0 degrees C. Results indicate that the thermosensitivity of erythrocytes from patients with type 1 diabetes is lower than for control individuals. Activation energies for lysis were similar for both control and 'diabetic' erythrocytes, being 298.3 and 287.7 kJ/mol, respectively. The steady-state fluorescence anisotropy measurement of TMA-DPH for each step of haemolysis was employed as a parameter characterizing membrane fluidity. We found that 'diabetic' erythrocyte membranes had significantly decreased fluidity. The relationship between fluidity and rate of haemolysis indicates that the rate-limiting step in the haemolysis reaction involves the rupturing of the membrane bilayer.
将人类红细胞暴露于高温下会导致细胞膜稳定性下降。测量了1型糖尿病患者和健康对照个体的红细胞在48.0至54.0摄氏度之间受热持续时间的函数关系下的热诱导溶血情况。结果表明，1型糖尿病患者红细胞的热敏感性低于对照个体。对照和“糖尿病”红细胞的裂解活化能相似，分别为298.3和287.7 kJ/mol。溶血各阶段TMA-DPH的稳态荧光各向异性测量被用作表征膜流动性的参数。我们发现“糖尿病”红细胞膜的流动性显著降低。流动性与溶血速率之间的关系表明，溶血反应中的限速步骤涉及膜双层的破裂。
