Tong P, Thomas T, Wilkinson R
Department of Medicine (Nephrology), University of Newcastle upon Tyne, United Kingdom.
Biochem Med Metab Biol. 1994 Aug;52(2):132-5. doi: 10.1006/bmmb.1994.1044.
The fluorescence anisotropy of 1,6-diphenylhexatriene (DPH) and trimethylammonium-DPH in the membranes of intact human erythrocytes and ghost membranes was compared. The anisotropy of fluorophores is significantly higher in intact erythrocytes compared to that in ghost membranes. Perturbation of membranes by heating at 47 degrees C and use of a rotating stirrer affected the anisotropy of fluorophores in intact erythrocytes only. These results suggest that: (a) spectrin has a significant modulating effect on membrane fluidity, and (b) the physical properties of the cell membrane are different between intact erythrocytes and erythrocyte ghost membranes.
比较了完整人红细胞膜和血影膜中1,6 - 二苯基己三烯（DPH）和三甲基铵 - DPH的荧光各向异性。与血影膜相比，完整红细胞中荧光团的各向异性显著更高。在47摄氏度加热以及使用旋转搅拌器对膜进行扰动，仅影响完整红细胞中荧光团的各向异性。这些结果表明：（a）血影蛋白对膜流动性具有显著的调节作用，以及（b）完整红细胞和红细胞血影膜之间细胞膜的物理性质有所不同。
