Wilson B W, Taylor R G, Fowler W M, Patterson G T, Nieberg P A, Linkhart S G, Linkhart T A, Fry D M
J Neurol Sci. 1975 Oct;26(2):133-46. doi: 10.1016/0022-510x(75)90026-x.
Fifty-nine biopsies of human muscle, 53 of them abnormal, 6 normal, were studied for the histochemical localization of acetylcholinesterase (AChE) using frozen sections and light microscopy. In addition to AChE which was found at the myoneural and myotendon junction, specific staining was found around the periphery of many fibers from normal and abnormal muscles. Moreover, AChE activity was found to be high in the sarcoplasm of more than 10% of the fibers from 28 biopsies of abnormal muscle including cases of hemiplegia, spinal cord injury, denervation and neuropathy, infantile spinal muscle atrophy, Duchenne, limb-girdle and facioscapulohumeral dystrophies, Schwartz-Jampel syndrome and a myasthenic syndrome. Of the muscles from experimental animals examined, only the Rhesus monkey exhibited AChE around the periphery of the fibers, and only the dystrophic chicken and not the dystrophic mouse or hamster, showed extensive sarcoplasmic AChE. Histograms of muscle fiber diameters indicated that AChE in the sarcoplasm was associated with fibers of all sizes, depending on the nature of the disorder examined. Fibers containing AChE were smaller than unstained fibers in dystrophic chicken muscle. The results suggest that in the human, sarcoplasmic AChE is reversibly repressed during muscle maturation and that its mode of regulation by motor neurons is similar to that found in the chicken.
对59份人体肌肉活检样本进行了研究，其中53份异常，6份正常，采用冰冻切片和光学显微镜观察乙酰胆碱酯酶（AChE）的组织化学定位。除了在肌神经和肌腱连接处发现AChE外，在正常和异常肌肉的许多纤维周边也发现了特异性染色。此外，在28份异常肌肉活检样本（包括偏瘫、脊髓损伤、去神经支配和神经病变、婴儿脊髓性肌萎缩、杜兴氏、肢带型和面肩肱型肌营养不良、施瓦茨-詹佩尔综合征和一种重症肌无力综合征病例）中，超过10%的纤维肌浆内AChE活性较高。在所检查的实验动物肌肉中，只有恒河猴在纤维周边表现出AChE，只有患营养不良症的鸡而非患营养不良症的小鼠或仓鼠，肌浆内有广泛的AChE。肌肉纤维直径直方图表明，肌浆内的AChE与各种大小的纤维相关，这取决于所检查疾病的性质。在患营养不良症的鸡肌肉中，含有AChE的纤维比未染色的纤维小。结果表明，在人类中，肌浆内AChE在肌肉成熟过程中受到可逆性抑制，并且其受运动神经元的调节方式与在鸡中发现的相似。
