Welder C A, Lee D H, Takei F
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada.
J Immunol. 1993 Mar 15;150(6):2203-10.
Murine recombinant soluble intercellular adhesion molecule-1 (sICAM-1) was produced and characterized. When immobilized on plastic microtiter wells, sICAM-1 efficiently mediated LFA-1-dependent cell adhesion, indicating that the purified protein retained the ability to bind to LFA-1. However, sICAM-1 in solution, at concentrations up to 100 micrograms/ml, was incapable of inhibiting the phorbol ester-induced homotypic aggregation of lymphocytes, the adhesion of T cells to plastic immobilized sICAM-1, and CTL effector function, all of which are mediated by intercellular adhesion molecule-1:LFA-1 interaction. In contrast, uniform polystyrene microspheres coated with sICAM-1 bound specifically to LFA-1+ cells and efficiently inhibited the adhesion of T cells to immobilized sICAM-1. The sICAM-1-coated microspheres also inhibited CTL function, but the inhibition was only partial. These results suggest that although monomeric sICAM-1 cannot competitively inhibit cell adhesion mediated by intercellular adhesion molecule-1 and LFA-1, microspheres coated with sICAM-1 can inhibit such cell adhesion.
制备并鉴定了小鼠重组可溶性细胞间黏附分子-1（sICAM-1）。当固定在塑料微量滴定板孔上时，sICAM-1能有效介导依赖淋巴细胞功能相关抗原-1（LFA-1）的细胞黏附，这表明纯化后的蛋白保留了与LFA-1结合的能力。然而，溶液中浓度高达100微克/毫升的sICAM-1无法抑制佛波酯诱导的淋巴细胞同型聚集、T细胞与固定在塑料上的sICAM-1的黏附以及细胞毒性T淋巴细胞（CTL）效应功能，所有这些都是由细胞间黏附分子-1与LFA-1相互作用介导的。相比之下，包被有sICAM-1的均匀聚苯乙烯微球能特异性结合LFA-1阳性细胞，并有效抑制T细胞与固定化sICAM-1的黏附。sICAM-1包被的微球也能抑制CTL功能，但这种抑制只是部分性的。这些结果表明，虽然单体sICAM-1不能竞争性抑制由细胞间黏附分子-1和LFA-1介导的细胞黏附，但包被有sICAM-1的微球可以抑制这种细胞黏附。
