Colthup P V, Young G C, Felgate C C
Drug Metabolism III Department, Glaxo Group Research Limited, Ware, Herts, U.K.
J Pharm Sci. 1993 Mar;82(3):323-5. doi: 10.1002/jps.2600820321.
Salmeterol (as the hydroxynaphthoate) is the first of a new class of long-acting beta-adrenergic receptor agonists with both bronchodilator and anti-inflammatory actions. A sensitive, accurate, and precise high-performance liquid chromatographic method for the determination of salmeterol in rat and dog plasma is described. Samples are prepared by solid-phase extraction and, after chromatography of the extracts on a reversed-phase styrene/divinylbenzene analytical column, salmeterol is detected by fluorescence monitoring (excitation wavelength, 230 nm; emission wavelength, 305 nm). The method is sensitive to 1 ng/mL, at which concentration the coefficient of variation was 16.3% in a single assay run. Repeated analyses of quality control samples, nominally at 2 ng/mL, were carried out over a number of assay runs with a coefficient of variation of 10.4%. The method is specific for salmeterol with respect to endogenous plasma components and identified metabolites. The assay was applied to the analysis of salmeterol in plasma of rats and dogs from pharmacokinetic studies.
沙美特罗（以羟萘甲酸盐形式）是一类新型长效β-肾上腺素能受体激动剂中的首个药物，具有支气管扩张和抗炎作用。本文描述了一种灵敏、准确且精密的高效液相色谱法，用于测定大鼠和犬血浆中的沙美特罗。样品通过固相萃取制备，提取物在反相苯乙烯/二乙烯苯分析柱上进行色谱分离后，通过荧光监测（激发波长230nm；发射波长305nm）检测沙美特罗。该方法对1ng/mL敏感，在此浓度下，单次测定运行的变异系数为16.3%。在多个测定运行中对名义浓度为2ng/mL的质量控制样品进行重复分析，变异系数为10.4%。该方法对于内源性血浆成分和已鉴定的代谢物而言对沙美特罗具有特异性。该测定法应用于药代动力学研究中大鼠和犬血浆中沙美特罗的分析。
