Kim K H, Yun H W, Kim H J, Park H J, Choi P W
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Arch Pharm Res. 1998 Apr;21(2):212-6. doi: 10.1007/BF02974030.
A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of salmeterol, S-(+)-salmeterol and R-(-)-salmeterol in urine. The salmeterol was separated from the interfering components in urine and quantified on the silica column, and the enantiomeric composition was determined on a Sumichiral OA-4700 chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the salmeterol in the eluent from the achiral column before backflushing onto the chiral phase. The coupled system was validated.
已开发出一种非手性-手性联用高效液相色谱系统，用于测定尿液中沙美特罗的对映体，即S-(+)-沙美特罗和R-(-)-沙美特罗。沙美特罗与尿液中的干扰成分分离，并在硅胶柱上进行定量，对映体组成在Sumichiral OA-4700手性固定相上测定。两根柱子通过配备硅胶预柱的切换阀连接。预柱用于在将非手性柱流出物反冲到手性相之前浓缩其中的沙美特罗。该联用系统经过了验证。
