Lee S H
Am J Clin Pathol. 1978 Aug;70(2):197-203. doi: 10.1093/ajcp/70.2.197.
A 17beta-estradiol-6-carboxymethyl-oxime-bovine serum albumin--fluorescein isothiocyanate conjugate is prepared by attaching on the average 11 moles of the fluorescein dye and 24 moles of the steroid hormone to each mole of the protein carrier. This fluorescent estradiol conjugate is used as a tracer to detect estrogen receptor of human mammary cancer cells in frozen sections. The cytochemical findings indicate that mammary carcinomas are composed of heterogeneous populations of receptor-positive and receptor-negative cancer cells in varying proportions and probably should be classified according to the percentages of receptor-positive cells in the cancer cell populations for better correlation with endocrine therapies.
通过将平均11摩尔荧光素染料和24摩尔甾体激素连接到每摩尔蛋白质载体上，制备出17β - 雌二醇 - 6 - 羧甲基 - 肟 - 牛血清白蛋白 - 异硫氰酸荧光素共轭物。这种荧光雌二醇共轭物用作示踪剂，以检测冰冻切片中人类乳腺癌细胞的雌激素受体。细胞化学研究结果表明，乳腺癌由比例各异的受体阳性和受体阴性癌细胞异质群体组成，为了与内分泌疗法有更好的相关性，可能应根据癌细胞群体中受体阳性细胞的百分比进行分类。
