肺癌中谷胱甘肽相关酶和增殖抗原的免疫组织化学研究。与顺铂敏感性的关系。

Immunohistochemical study of glutathione-related enzymes and proliferative antigens in lung cancer. Relation to cisplatin sensitivity.

作者信息

Ogawa J, Iwazaki M, Inoue H, Koide S, Shohtsu A

机构信息

First Department of Surgery, Tokai University School of Medicine, Isehara, Japan.

出版信息

Cancer. 1993 Apr 1;71(7):2204-9. doi: 10.1002/1097-0142(19930401)71:7<2204::aid-cncr2820710707>3.0.co;2-o.

DOI:10.1002/1097-0142(19930401)71:7<2204::aid-cncr2820710707>3.0.co;2-o

PMID:8095847

Abstract

BACKGROUND

With resected tumor tissue from 84 patients with lung cancer, the expression of glutathione peroxidase (GPX), glutathione reductase (GR), proliferating cell nuclear antigen (PCNA), and epidermal growth factor receptor (EGFR) was examined in relation to cisdiamminedichloroplatinum (CDDP) sensitivity.

METHODS

The CDDP sensitivity was assessed from an increase of cells in the S-phase or G2M-phase by a DNA histogram after the tumor cells were incubated in a CDDP solution. The expression of GPX, GR, PCNA, and EGFR for each tumor was studied with an indirect immunoperoxidase technique on paraffin-embedded tissues.

RESULTS

The percentage of patients sensitive to CDDP according to the histologic type was 86% for small cell carcinomas, 40% for large cell carcinomas, 31% for squamous cell carcinomas, and 6% for adenocarcinomas. There was an inverse relationship between CDDP sensitivity and the frequency of GPX and GR expression, and both were significantly different among histologic types. The GPX and GR expression was significantly lower in the CDDP-sensitive group than in the resistant group (P < 0.01). However, the expression of PCNA and EGFR was significantly lower in the sensitive group in non-small cell carcinomas and squamous cell carcinomas (P < 0.05).

CONCLUSIONS

From the above findings, an immunohistochemical study of these antigens may be useful for predicting CDDP sensitivity in lung cancer.

摘要

背景

利用84例肺癌患者的肿瘤切除组织，检测了谷胱甘肽过氧化物酶（GPX）、谷胱甘肽还原酶（GR）、增殖细胞核抗原（PCNA）和表皮生长因子受体（EGFR）的表达与顺二氯二氨铂（CDDP）敏感性的关系。

方法

在肿瘤细胞于CDDP溶液中孵育后，通过DNA直方图中S期或G2M期细胞的增加来评估CDDP敏感性。采用间接免疫过氧化物酶技术对石蜡包埋组织中各肿瘤的GPX、GR、PCNA和EGFR表达进行研究。

结果

根据组织学类型，对CDDP敏感的患者百分比分别为：小细胞癌86%，大细胞癌40%，鳞状细胞癌31%，腺癌6%。CDDP敏感性与GPX和GR表达频率呈负相关，且在不同组织学类型之间均有显著差异。CDDP敏感组的GPX和GR表达明显低于耐药组（P < 0.01）。然而，在非小细胞癌和鳞状细胞癌中，敏感组的PCNA和EGFR表达明显较低（P < 0.05）。