Akimoto J, Itoh H, Miwa T, Ikeda K
Department of Neurosurgery, Tokyo Medical College, Japan.
Brain Res Dev Brain Res. 1993 Mar 19;72(1):9-14. doi: 10.1016/0165-3806(93)90154-3.
Early glial development in the brains of rat embryos was immunohistochemically studied using antibodies to two kinds of glial-specific proteins, glutamine synthetase (GS) and glial fibrillary acidic protein (GFAP). The results showed that GS was first expressed in the ependymal lining of the ventral side of the neural tube in the cervical segment on Embryonic Day 14 and then in the radial glia radiating from the neural tube on Embryonic Day 16. Subsequently, GS-positive glia cells increased in number with time. On the other hand, GFAP was first expressed in the fimbria hippocampi and the pial surface on Embryonic Day 18, but no marked evidence of GFAP expression was noted in the GS-positive radial glia. These results suggested that a specific protein is expressed at each stage of early glial development. Since the radial glia was positive for GS, it was thought that GS-mediated neuronal-glial interactions may play an important role in the development of the central nervous system.
利用针对两种神经胶质特异性蛋白——谷氨酰胺合成酶（GS）和神经胶质纤维酸性蛋白（GFAP）的抗体，对大鼠胚胎大脑早期神经胶质发育进行了免疫组织化学研究。结果显示，GS于胚胎第14天首先在颈段神经管腹侧的室管膜内衬中表达，然后在胚胎第16天于从神经管放射状发出的放射状胶质细胞中表达。随后，GS阳性胶质细胞数量随时间增加。另一方面，GFAP于胚胎第18天首先在海马伞和软膜表面表达，但在GS阳性的放射状胶质细胞中未观察到明显的GFAP表达证据。这些结果表明，在神经胶质早期发育的每个阶段都有特定蛋白质表达。由于放射状胶质细胞对GS呈阳性，因此认为GS介导的神经元-神经胶质相互作用可能在中枢神经系统发育中起重要作用。
