Kittlesen D J, Brown L R, Braciale V L, Sambrook J P, Gething M J, Braciale T J
Beirne B. Carter Center for Immunology Research, University of Virginia Health Sciences Center, Charlottesville 22908.
J Exp Med. 1993 Apr 1;177(4):1021-30. doi: 10.1084/jem.177.4.1021.
Human lymphoblastoid cells transiently expressing the hemagglutinin (HA) glycoprotein of influenza virus are rapidly and efficiently recognized by CD4+ HA-specific T lymphocytes. This endogenous presentation pathway is sensitive to chloroquine and is therefore likely related to the classical class II major histocompatibility complex (MHC) exogenous pathway of antigen presentation. In this study we have examined a series of transport-defective HA mutants. We correlate the intracellular fate of the native antigen with its presentation characteristics. We have found that the native antigen must enter the secretory pathway since a cytosolic form is not presented. However, surface expression and normal trafficking through the Golgi apparatus are not required for efficient presentation. Instead, escape of native antigen from the endoplasmic reticulum appears to be both necessary and sufficient for gaining access to a compartment where antigen is processed and binds class II MHC molecules.
瞬时表达流感病毒血凝素（HA）糖蛋白的人淋巴母细胞可被CD4⁺HA特异性T淋巴细胞快速且高效地识别。这种内源性呈递途径对氯喹敏感，因此可能与经典的II类主要组织相容性复合体（MHC）外源性抗原呈递途径相关。在本研究中，我们检测了一系列运输缺陷型HA突变体。我们将天然抗原的细胞内命运与其呈递特征相关联。我们发现天然抗原必须进入分泌途径，因为胞质形式的抗原不会被呈递。然而，有效呈递并不需要表面表达和通过高尔基体的正常运输。相反，天然抗原从内质网逃逸似乎对于进入一个抗原被加工并结合II类MHC分子的区室而言既是必要的也是充分的。
