Taylor K A, Paskewitz S M, Copeland R S, Koros J, Beach R F, Githure J I, Collins F H
Centers for Disease Control-Kenya, Nairobi.
J Med Entomol. 1993 Mar;30(2):457-61. doi: 10.1093/jmedent/30.2.457.
Two DNA-based methods, the restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR), were used to identify mosquitoes of the Anopheles gambiae Giles complex collected in Kenya. Field-collected specimens of An. gambiae, An. arabiensis Patton, and An. merus Donity were tested. From a sample of 208 mosquitoes, 181 (87%) were identified by the RFLP method and 205 (99%) were identified by the PCR method. There was complete concordance between the two methods with regard to species identification. PCR assays were simpler, faster, and more reliable than RFLP assays.
采用两种基于DNA的方法，即限制性片段长度多态性（RFLP）和聚合酶链反应（PCR），对在肯尼亚采集的冈比亚按蚊复合体的蚊子进行鉴定。对野外采集的冈比亚按蚊、阿拉伯按蚊和梅氏按蚊标本进行了检测。在208只蚊子样本中，RFLP方法鉴定出181只（87%），PCR方法鉴定出205只（99%）。在物种鉴定方面，两种方法完全一致。PCR检测比RFLP检测更简单、更快且更可靠。
