Serra D, Asins G, Hegardt F G
Unit of Biochemistry, School of Pharmacy, University of Barcelona, Spain.
Arch Biochem Biophys. 1993 Mar;301(2):445-8. doi: 10.1006/abbi.1993.1169.
The ketogenic mitochondrial 3-hydroxy 3-methylglutaryl-coenzyme A (HMG-CoA) synthase gene is expressed in intestine of suckling rats, its mRNA levels changing with age. Intestine mitochondrial mRNA values reach maximum levels on the 12th postnatal day and then decrease smoothly. Mother's milk may influence the intestine expression, since mRNA levels at birth are very low, increasing after the first lactation. Moreover, rats weaned at either Day 18 or 21 decrease their mRNA levels dramatically and there is no expression in adult rats. Mitochondrial HMG-CoA synthase is also expressed in liver of suckling rats but the developmental pattern of mRNAs is different from that in intestine, showing the highest values at Day 3 of life. mRNA levels in liver are lower than in intestine for most of the suckling period, suggesting the physiological relevance of the intestine for the ketogenic process of the whole body. Liver mRNA levels on weaning and in adult rats are high enough to sustain hepatic ketogenesis.
生酮线粒体3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）合酶基因在乳鼠肠道中表达，其mRNA水平随年龄变化。肠道线粒体mRNA值在出生后第12天达到最高水平，然后平稳下降。母乳可能会影响肠道表达，因为出生时mRNA水平非常低，首次哺乳后会升高。此外，在第18天或第21天断奶的大鼠其mRNA水平会显著下降，成年大鼠中则没有表达。线粒体HMG-CoA合酶也在乳鼠肝脏中表达，但mRNA的发育模式与肠道不同，在出生后第3天显示出最高值。在大多数哺乳期间，肝脏中的mRNA水平低于肠道，这表明肠道对全身生酮过程具有生理相关性。断奶和成年大鼠肝脏中的mRNA水平足以维持肝脏生酮作用。
