Sentsova O Iu, Nikitina K A, Gusev M V
Mikrobiologiia. 1975 Jul-Aug;44(4):595-9.
The content of ATP in the cells of Anabaena variabilis does not change during one day in the darkness as compared to that in the cells grown in the light, this suggesting the endogenous energy reserve of the cell. The level of ATP gradually decreases when the cells are incubated in the darkness during 25 days, but does not reach zero. The content of ATP increases for a short period in the light in the cells that have been incubated in the darkness during several days (up to 7 days). On the contrary, the level of ATP increases to a small extent and then falls in the cells grown in the light. These differences suggest disturbed coupling of the synthesis and utilization of ATP in the cells grown in the darkness. The content of ATP increases again in the darkness, confirming that the alga is capable of synthesizing ATP in the darkness.
与在光照下生长的细胞相比,可变鱼腥藻细胞中的ATP含量在黑暗中一天内没有变化,这表明细胞具有内源性能量储备。当细胞在黑暗中培养25天时,ATP水平逐渐下降,但未降至零。在黑暗中培养数天(长达7天)的细胞在光照下短时间内ATP含量会增加。相反,在光照下生长的细胞中,ATP水平先略有上升然后下降。这些差异表明在黑暗中生长的细胞中ATP的合成与利用的偶联受到干扰。ATP含量在黑暗中再次增加,证实该藻类能够在黑暗中合成ATP。