枯草芽孢杆菌在噬菌体phi 29头部组装方面存在缺陷的突变体。

Bacillus subtilis mutants defective in bacteriophage phi 29 head assembly.

作者信息

Rajagopal B S, Reilly B E, Anderson D L

机构信息

Department of Microbiology, University of Minnesota, Minneapolis 55455.

出版信息

J Bacteriol. 1993 Apr;175(8):2357-62. doi: 10.1128/jb.175.8.2357-2362.1993.

DOI:10.1128/jb.175.8.2357-2362.1993

PMID:8096839

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204524/

Abstract

Virus assembly mutants of asporogenous Bacillus subtilis defective in bacteriophage phi 29 head assembly were detected by the use of antibodies that reacted strongly with the free dodecameric phi 29 portal vertex composed of gene product 10 (gp10) but weakly with the portal vertex assembled into proheads or phage. Phage adsorption and the synthesis of phage proteins, DNA-gene product 3, and prohead RNA were normal in these mutants, but prohead and phage production was greatly reduced. The assembly defect was transferred to competent B. subtilis by transformation and transduction. PBS1 transduction showed that the vam locus was linked to Tn917 located at 317 degrees on the B. subtilis chromosome.

摘要

利用与由基因产物10（gp10）组成的游离十二聚体噬菌体φ29门户顶点强烈反应但与组装到原头部或噬菌体中的门户顶点反应较弱的抗体，检测了在噬菌体φ29头部组装中存在缺陷的产孢缺陷型枯草芽孢杆菌的病毒组装突变体。在这些突变体中，噬菌体吸附以及噬菌体蛋白质、DNA-基因产物3和原头部RNA的合成均正常，但原头部和噬菌体的产生大大减少。通过转化和转导将组装缺陷转移到感受态枯草芽孢杆菌中。PBS1转导表明，vam基因座与位于枯草芽孢杆菌染色体317°处的Tn917连锁。