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枯草芽孢杆菌中调控噬菌体抗性的基因的染色体定位。

Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis.

作者信息

Young F E, Smith C, Reilly B E

出版信息

J Bacteriol. 1969 Jun;98(3):1087-97. doi: 10.1128/jb.98.3.1087-1097.1969.

Abstract

Many of the viruses which infect Bacillus subtilis require glucosylated polyglycerol teichoic acid for adsorption. These mutants can be divided into three classes on the basis of enzymatic defects and growth on galactose-minimal medium. Transduction with phage PBS1 reveals that two of these, gtaA and gtaB, are linked to hisA1, whereas the gtaC locus is linked to argC. Analysis by deoxyribonucleic acid-mediated transformation indicates that these loci exist in a cluster between the hisA1 and argC4 loci. Anomalies in mapping in the group II region of the chromosome exist. The basis of these anomalies is discussed.

摘要

许多感染枯草芽孢杆菌的病毒需要糖基化的聚甘油磷壁酸来进行吸附。这些突变体可根据酶缺陷以及在半乳糖基本培养基上的生长情况分为三类。用噬菌体PBS1进行转导表明,其中两个,即gtaA和gtaB,与hisA1连锁,而gtaC位点与argC连锁。通过脱氧核糖核酸介导的转化分析表明,这些位点存在于hisA1和argC4位点之间的一个簇中。在染色体的II组区域存在定位异常。讨论了这些异常的基础。

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